@article{326,
  author = {Ferenc Zsila and E Hazai and L Sawyer},
  title = {Binding of the pepper alkaloid piperine to bovine \^I˛-lactoglobulin: Circular dichroism spectroscopy and molecular modeling study},
  abstract = {<p>The pepper alkaloid piperine is a nontoxic, natural dietary compound with a broad range of physiological activity. The present work is the first demonstration of its interaction with a mammalian protein. Circular dichroism (CD) spectroscopy was used to reveal and analyze the binding of piperine to a lipocalin protein. Induced CD spectra measured in pH 7.7 phosphate buffer at 37\^A°C demonstrated reversible, non-covalent association of piperine with bovine \^I˛-lactoglobulin (BLG), the major whey protein in milk. The binding parameters (Ka \^a\textperthousandˆ 8 \u A— 104 M-1</p>},
  year = {2005},
  journal = {Journal of Agricultural and Food Chemistry},
  volume = {53},
  pages = {10179-10185},
  url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-30544433324&partnerID=40&md5=7000d9382db7a91494e08da756033c0f},
  doi = {10.1021/jf051944g},
}