@article{129,
  keywords = {animal cell, animal experiment, animal model, Article, biomechanics, Body fluids, bone, bone defect, bone densitometry, bone density, Bone formation, bone mass, bone matrix, Bone replacement, bone transplantation, Breaking force, calvaria defect, cell adhesion, cell function, Cells, Coatings, Computerized tomography, Control groups, controlled study, Cytology, DBM, Defects, Demineralized bone matrix, ex vivo study, force, in vitro study, in vivo study, male, micro-computed tomography, Nonhealing defects, nonhuman, ossification, postoperative period, rat, rigidity, Serum Albumin, skull defect, stem cell, stem cells, tissue graft},
  author = {D.B. Horváthy and G. Vácz and T. Szabó and Imola Szigyártó and I. Torő and B. Vámos and I. Hornyák and K. Renner and T. Klára and B.T. Szabó and C. Dobő-Nagy and A. Doros and Z. Lacza},
  title = {Serum albumin coating of demineralized bone matrix results in stronger new bone formation},
  abstract = {<p>Blood serum fractions are hotly debated adjuvants in bone replacement therapies. In the present experiment, we coated demineralized bone matrices (DBM) with serum albumin and investigated stem cell attachment in vitro and bone formation in a rat calvaria defect model. In the in vitro experiments, we observed that significantly more cells adhere to the serum albumin coated DBMS at every time point. In vivo bone formation with albumin coated and uncoated DBM was monitored biweekly by computed tomography until 11 weeks postoperatively while empty defects served as controls. By the seventh week, the bone defect in the albumin group was almost completely closed (remaining defect 3.0 ± 2.3%), while uncoated DBM and unfilled control groups still had significant defects (uncoated: 40.2 ± 9.1%, control: 52.4 ± 8.9%). Higher density values were also observed in the albumin coated DBM group. In addition, the serum albumin enhanced group showed significantly higher volume of newly formed bone in the microCT analysis and produced significantly higher breaking force and stiffness compared to the uncoated grafts (peak breaking force: uncoated: 15.7 ± 4 N, albumin 46.1 ± 11 N). In conclusion, this investigation shows that implanting serum albumin coated DBM significantly reduces healing period in nonhealing defects and results in mechanically stronger bone. These results also support the idea that serum albumin coating provides a convenient milieu for stem cell function, and a much improved bone grafting success can be achieved without the use of exogenous stem cells. © 2015 Wiley Periodicals, Inc.</p>},
  year = {2016},
  journal = {Journal of Biomedical Materials Research - Part B Applied Biomaterials},
  volume = {104},
  pages = {126-132},
  month = {2016},
  publisher = {John Wiley and Sons Inc.},
  isbn = {15524973 (ISSN)},
  url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84952876941&partnerID=40&md5=3f3642dd5c86c82ce98043ed66033cfb},
  note = {Export Date: 7 March 2016CODEN: JBMRGCorrespondence Address: Horváthy, D.B.; Institute of Human Physiology and Clinical Experimental Research, Semmelweis UniversityHungary; email: denes.horvathy@tissueengineering.huChemicals/CAS: serum albumin, 9048-46-8References: Holzmann, P., Niculescu-Morzsa, E., Zwickl, H., Halbwirth, F., Pichler, M., Matzner, M., Gottsauner-Wolf, F., Nehrer, S., Investigation of bone allografts representing different steps of the bone bank procedure using the CAM-model (2010) ALTEX, 27, pp. 97-103;Alaee, F., Hong, S.H., Dukas, A.G., Pensak, M.J., Rowe, D.W., Lieberman, J.R., Evaluation of osteogenic cell differentiation in response to bone morphogenetic protein or demineralized bone matrix in a critical sized defect model using GFP reporter mice (2014) J Orthop Res, 32, pp. 1120-1128; Hussain, A., Takahashi, K., Sonobe, J., Tabata, Y., Bessho, K., Bone regeneration of rat calvarial defect by magnesium calcium phosphate gelatin scaffolds with or without bone morphogenetic protein-2 (2014) J Maxillofac Oral Surg, 13, pp. 29-35; Liu, T., Wu, G., Wismeijer, D., Gu, Z., Liu, Y., Deproteinized bovine bone functionalized with the slow delivery of BMP-2 for the repair of critical-sized bone defects in sheep (2013) Bone, 56, pp. 110-118; Even, J., Eskander, M., Kang, J., Bone morphogenetic protein in spine surgery: Current and future uses (2012) J Am Acad Orthop Surg, 20, pp. 547-552; Fu, R., Selph, S., McDonagh, M., Peterson, K., Tiwari, A., Chou, R., Helfand, M., Effectiveness and harms of recombinant human bone morphogenetic protein-2 in spine fusiona systematic review and meta-analysis (2013) Ann Intern Med, 158, pp. 890-902; Malhotra, A., Pelletier, M.H., Yu, Y., Walsh, W.R., Can platelet-rich plasma (PRP) improve bone healing? 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}